重组III型三螺旋胶原蛋白白皮书

◼IIIIIIIII•28III•IIIIII•IIIIII•IIIIIIIIIIIIIII◼29GMPGSPIIIⅢⅢⅢAIⅢⅢ——CEO ◼IIIIIIIIIDNAIIIIII01IIIIIIIIIIIIIII——◼◼◆◆◆◼011.1.1 CollagenCollagen peptideCollagenTropocollagen3ααα1α2α3IIIα1α1(III)III[α1(III)]3ααIII 138,000 Da1.1.2 1.1 3αG-X-YIIIXXIIIIIXII / XIVV Bielajew BJ, Hu JC, Athanasiou KA. Collagen: quantification, biomechanics, and role of minor subtypes in cartilage. Nat Rev Mater. 2020 Oct;5(10):730-747. doi: 10.1038/s41578-020-0213-1. Epub 2020 Jul 20.1.2 28FACITMACITs 41.2.1 α—α20α1,000284IIIIIIVXIXXIVXXVIIIII III V XI III III XXIV XXVII IIIV XI C N FACITIXXIIXIVXVIXIXXXIIFACIT IVVIIXXVIIIIV VII MACITsXIIIXXIIIXXVXIIIXXIII XXV II 11N 31/2αICOL1A1[α1(I)]2α2(I)67 nm COL1A2[α1(I)]3IICOL2A1[α1(II)]3IIICOL3A1[α1(III)]3VCOL5A1[α1(V)]2α2(V)9 nmCOL5A2[α1(V)α2(V)α3(V)]COL5A3[α1(V)]3XICOL11A1[α1(XI)α2(XI)α3(XI)]V COL11A2XXIVCOL24A1[α1(XXIV)]3XXVIICOL27A1[α1(XXVII)]34 I II III I II III IV 2/21.2 28FACITMACITs 4αFACITIXCOL9A1[a1(IX)a2(IX) a3(IX)]II COL9A2COL9A3XIICOL12A1[a1(XII)]3I XIVCOL14A1[a1(XIV)]3 I II XVICOL16A1a1(XVI)-1 D II XIXCOL19A1a1(XIX)N-[a1(XIX)]2[a1(XIX)]3[a1(XIX)]4[a1(XIX)]5[a1(XIX)]6XXCOL20A1a1(XX)XXIICOL22A1a1(XXII)-IVCOL4A1[a1(IV)]2a2(IV)COL4A2[a1(IV)]2a3(IV)COL4A3[a1(IV)]2a4(IV)COL4A4[a1(IV)]2a5(IV)COL4A5[a1(IV)]2a6(IV) VIICOL7A1[a1(VII)]3XVIIICOL18A1a1(XVIII) MACITsXIIICOL13A1 a1(XIII)XXIIICOL23A1a1(XXIII)XXVCOL25A1 a1(XXV)R XXVICOL26A1a1(XXVI) XVIIICOL18A1a1(XVIII) XVCOL15A1 a1(XV) XXVIIICOL28A1 a1(XXVIII) 1.3 III 1971MillerIII III III 1.3.1 IIIIII 1971MillerIII -1971MillerIII 1989Tromp, GIII -Ehlers-Danlos Syndrome, EDS——III EDS1991III III III 1980s ~ 1990sDNA~19941997KlugCummings KlugCummingsIIIIII67nm2005 2021III III1.3.2 III III 243αIII CNIII Kuivaniemi H, Tromp G. Type III collagen (COL3A1): Gene and protein structure, tissue distribution, and associated diseases. Gene. 2019 Jul 30;707:151-171. doi: 10.1016/j.gene.2019.05.003. Epub 2019 May 7. PMID: 31075413; PMCID: PMC6579750.III αIII 1,068400 kDaIII343Gly-X-Y XYXProYHypHylPP II α-X-YXYααN-C-1.3 III subunitαIIPP IIPP II 3.30.2860.858III 1.3.3 III III pre-procollagenprocollagenIII procollagenCsuperhelixRNAmRNA1,000 endoplasmic reticulumER•prolyl hydroxylaselysyl hydroxylase•5-•239145-4-4-•CNtropocollagenIII •4-3-4-3-•C-αIIIC-81N-C-III C-C-1.3 III 1.3.4 III III I /III Fagerberg20149527RNAmRNACOL3A1 mRNAIII -E7.5E17.5RNAI III 1991KeeneIII 30~80III 1984d‘ArdenneIII III III 1.3 III III Kuivaniemi H, Tromp G. Type III collagen (COL3A1): Gene and protein structure, tissue distribution, and associated diseases. Gene. 2019 Jul 30;707:151-171. doi: 10.1016/j.gene.2019.05.003. Epub 2019 May 7. PMID: 31075413; PMCID: PMC6579750.•COL3A1 mRNAFaderberg(Fagerberg et al., 2014)RNA•RPKM: Reads Per Kilobase per Million mapped readsRPKMIII 02III2.1 2.1.1 /%/℃HypPro10.1411.9538.96.8511.1037.09.4012.1036.39.7012.3035.89.0512.6435.08.7211.7934.06.9011.5024.65.609.1015.9HypPro LINZEUGOLISYOSHIMURA2.2 III III 2.2.1 III70%III III III 264.71279.12241.79209.5278.87123.2798.4171.3IIIIIIII I II